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This triple therapy [2] approach leverages the complementary actions of GLP-1, GIP, and glucagon receptors to produce a more comprehensive and effective treatment compared to single or dual agonist drugs

First, the clinical toxicity profile is transformed rather than eliminated, as seen in the hepatotoxicity of MEDI4276 and keratitis with ZW49

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doi: 10.2337/dc100704 54 WasadaTMcCorkleKHarrisVKawaiKHowardBUngerRH

26 During retatrutide escalation, monthly monitoring of heart rate, fasting glucose, and GI symptom severity is appropriate at each dose step, given the three-receptor mechanism and the longer exposure window before maintenance dose is reached

Five days later, the mice were euthanized, and their lungs were harvested, rinsed with cold PBS, snap-frozen in liquid nitrogen, and stored at 80 C until further analysis