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The process was standardized as follows: At 37C during the whole night, 100 mg of adipose tissue was incubated in 700 L of an X-gal-containing staining solution

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The critical difference between the two forms comes down to a single molecular addition: the Drug Affinity Complex (DAC)

The colour change typically resolves within a few days to weeks but has been reported up to 35 days post administration

Molecular Pharmacology

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