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Peptide mass: 10mg Bacteriostatic water: 2mL Concentration: 10mg 2mL = 5 mg/mL = 5,000 mcg/mL Peptide per unit (100-unit syringe): 5 mg 100 = 0.05 mg/unit = 50 mcg/unit If research protocol calls for 250 mcg per research unit: 250 mcg 50 mcg/unit = 5 units on the syringe Total doses per vial at 250 mcg each: 10,000 mcg 250 mcg = 40 research doses That single 10mg vial, reconstituted with 2mL of bacteriostatic water, provides 40 research doses of 250 mcg each, drawn to the 5-unit mark on a standard insulin syringe

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The injection volume was adjusted according to body weight using a proportional dosing rule based on a reference volume of 100 L for a 30 g animal