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The morning after mating, a vaginal wash was performed to check the presence of sperm

Samples were then amplified and sequenced on a NextSeq 500 system (Illumina), where an average of 30 million reads were generated from each sample

Beyond that, the evidence is per-compound plus the CagriSema (cag + sema) Phase 3 data as the closest human analog

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have confirmed that the increase of circulating TMAO downregulates IL-10 and promotes vascular inflammation, contributing to endothelial dysfunction by mouse experiments (55)