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Evidence type: Human RCT | Observational | Animal | In vitro | Regulatory Mechanism of Action 5-Amino-1MQ works by blocking the NNMT enzyme , which is responsible for consuming nicotinamide and depleting NAD+ stores

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Supplementary Validation Method: LCMS/MS For high-sensitivity detection requirements, liquid chromatographytandem mass spectrometry is used for supplementary validation, especially suitable for trace component analysis and metabolic studies

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