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The cells were maintained in 5% CO2 at 37C in RPMI-1640 medium (Thermo Fisher Scientific), supplemented with 10% fetal bovine serum (Thermo Fisher Scientific), 1% HEPES (Gibco), 1% Pen-Strep (Gibco), and 2.2% glucose (Gibco)

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Further Reading: Calcium AKG Anti-Ageing Benefits End Note Glutathione is a powerful antioxidant needed for maintaining health and fighting oxidative stress

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