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Proteins with an N-terminal his tag (GSTs A1-1, A2-2, A3-3, P1-1, and S1-1) were purified as follows

Autophagic flux measurement Autophagic flux was measured in cells transfected with Autophagy Tandem Sensor GFP-mRFP-LC3B

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An analysis of variance taking age into consideration did not change the TIMP-1 finding ( p = 0.463)