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Finding the balance between efficacy and tolerability drove much of the development work for both molecules

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1 g RNA was reverse transcribed into cDNA using the Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT, Promega) following the manufacturers instructions

John Salerno designed his Chelation IV treatment with Dimercaptopropane Sulfonic Acid, to help patients eliminate heavy metals, such as lead, mercury, arsenic, and cadmium, that can accumulate in the body due to environmental exposure, occupational hazards, or dietary sources