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The remaining fat particles were digested with 0.2% collagenase (Gibco, Billings, MT, USA) in a water bath shaker at 37 C for 30 min, and the mixture was centrifuged at 1000 rpm for 5 min to remove undigested fat

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Pharmaceutical-grade API for some of these peptides do not yet exist at commercial scale, in large part because these substances have not been authorized for human use