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The choice of diluent is determined by the trial sponsor, the investigational medicinal product dossier (IMPD), and the site's aseptic preparation policy not by individual preference

To further enhance the BBB properties of the models, the medium was supplemented with cARLA (250 M 8-(4-chlorophenylthio)adenosine 3,5-cyclic monophosphate sodium salt, 17.5 M Ro-20-1724, 3 mM LiCl, 3 M A83-01

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Zhu J, Li J, Yang L, Liu S

transfer to -20 C or below on arrival

Avoid extrapolating animal dose by body weight without clinical guidance