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1f), 1 M FAM-labeled BRD4 BD1 was incubated with 3 M WDR26 CTLH or YPEL5WDR26 CTLH in the presence of 10 M JQ1 (Sigma-Aldrich) or ZZ1 (at 2% final DMSO concentration) for 1 h in buffer A

The conjugation process involves 6 major metabolic pathways, including methylation, sulfation, glucuronidation, glutathione conjugation, acetylation, and glycination

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Acetate is then broken down further into water and carbon dioxide, allowing the body to expel it