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However, despite affinity, albeit lower, of tirzepatide for the GLP-1 receptor, tirzepatide had no effect on body weight, food intake, fasting insulin, endogenous glucose production and insulin-stimulated glucose uptake in skeletal muscle and subcutaneous white adipose tissue of GLP-1 receptor knockout mice [89]

Box plot statistics for panels c , e , and f are reported in the source data

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doi: 10.1371/journal.pone.0182835 39 BrtkoJDvorakZ

The concept of "best time to apply" becomes less about pharmacokinetic optimization and more about practical convenience and skin site rotation