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The proteins were embedded in a box of OPC water molecules (minimum distance of 12 from the solute to the boundaries of the box) and the net charge of the system was set to zero by neutralization with Cl-ions using the program tLEaP included in AmberTools21 ( 60

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Healthcare providers may recommend or prescribe higher doses of B12 to treat a deficiency

The serum can be stored for one year at -20 C