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All cell lines except hTERT RPE-1 cells were cultured in DMEM (4.5 g l 1 glucose, Thermo Fisher Scientific) supplemented with 10% (v/v) FBS (Thermo Fisher Scientific), 100 U ml 1 penicillin (Thermo Fisher Scientific) and 100 g ml 1 streptomycin (Thermo Fisher Scientific)
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-carotene, -carotene, -carotene, and -cryptoxanthin are the most representative carotenoids
A strain with an add (encoding adenosine deaminase) mutation showed enhanced GSH production
Do not leave the vial in a car, near a stove, or in any place where it could be exposed to high temperatures
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