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The deficiency can arise due to improper diet or the inability to absorb the vitamin through natural means

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In brief, the proteins were precipitated with 0.5 mL ice cold 10% 5-sulfosalicylic acid and the tubes were incubated on ice for 10 min, centrifuged (Sorvall Instruments RC5C, DuPont, Minnesota, USA) for 15 min at 15,000 rpm at 4C

Your APRN sets the oral dose at intake based on your specific GI indication