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The ROS generation was detected with a Leica fluorescence microscope (Leica DM6000B) using Leica LAS X software, and quantitative analysis was performed with ImageJ software (version 1.60

Sermorelin wins on simplicity

The unpaired Student t test for comparison between two groups was used to determinate statistical significance

Mantle TJ et al

The author is also grateful to Professor Jon Fraenkel for DSIP data from 1984 to 2010, adapted from The political economy of economic reform in the Pacific