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The activity of the antioxidant enzyme GPx was determined by standard protocols, in blood samples (2 ml each), collected from all subjects, after the onset of arrhythmia (for groups I and II), by venipuncture (in K2EDTA vacutainers), using a standardized Ransel Laboratory kit (RandoxLaboratories, Ltd.)
Direct the stream of solvent toward the glass wall of the vial, not directly onto the lyophilized powder
The non-enzymatic components include the major cellular redox buffers: ascorbate (AsA) and glutathione (-glutamyl-cysteinyl-glycine, GSH) as well as tocopherol, flavonoids, alkaloids, and carotenoids (Arora et al., 2005)
A.ChenJ.Rakhmawati EmrilD.Fernndez-VillacortaF
Refrigeration 28C (3646F) for short-term use (up to ~3 months)