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Other assays Various in vitro cellular functional assays, gene/protein detections, and biochemical assays were performed as previously described in detail [23, 24], including assessments of cell proliferation (EdU-nuclear staining), migration (Transwell assay), invasion (Matrigel Transwell assay), capillary tube formation, reactive oxygen species (ROS) production (through MitoSOX staining), mitochondrial depolarization (JC-1 staining), Caspase-3 and Caspase-9 activity, TUNEL-nuclear staining, mitochondrial Complex I activity, and cellular ATP levels
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Compared to continued use of GLP-1s for three years, which corresponded to an 18% risk reduction, GLP-1 users who experienced interruption and subsequently resumed gained a 12% risk reduction on average
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