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The samples were centrifuged at 1000 rpm for 3 min using a KUBOTA 3520 (KUBOTA, Tokyo, Japan), and absorbance was measured at 595 nm using a GeneQuant 1300 spectrophotometer (Biochrome, Cambridge, UK)

Signs you may need to decrease dose: persistent injection site reactions, nausea, fatigue, or excessive skin sensitivity

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Bioflavonoids are important both before and during binding

Kim P et al

Nonetheless, the present study does not directly identify the chemical modification, the modified residues/bonds, or the structural mechanism, all of which await further study