HDM antigens commonly produce allergic lung inflammation, and as such, are commonly used in mice to model human asthma [1]
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To immunoprecipitate DAT-GFP, the cell homogenates were incubated overnight at 4 C with a monoclonal anti-GFP antibody (G-6539, 1:100), followed by the addition of 50 l of a mixture of protein A and protein G Sepharose beads (2 h at 4 C in rotatory shaker)
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This dual mechanism may explain the absence of early-worsening effects and the apparent protective association observed in our real-world cohort. They concluded that prospective studies that incorporate standardized retinal imaging and extend follow-up are needed to confirm the durability of this benefit and to refine recommendations for patients considering weight loss medications who are at risk for DR
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