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Two types of TSs were generated: (i) TS-1, a simple insertion of an elastic linker (GPGGA) 8 at Tyr145 of EGFP, where GFP has been shown to tolerate the insertion of the full protein 1,14 , and (ii) TS-2, cpEGFP with the same elastic linker 14 (Fig

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Check for cloudiness, visible particles, or changes in solution consistency after thawing