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The Window Is Short The Bridge is a demonstration program with a fixed end date (although that end date has already been pushed out)

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To calibrate the probe, fully oxidized and fully reduced samples were generated by the addition of 20 mM N , N , N , N -Tetramethylazodicarboxamide (diamide, Sigma Aldrich ) or 100 mM Dithiotreitol (DTT, AppliChem ), respectively

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Upon release, Nrf2 translocates to the nucleus where it can bind to the antioxidant response element (ARE) located in the promoter region of genes coding for antioxidant enzymes and scavengers