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Unless indicated otherwise, cystine-free medium was prepared by supplementing cystine-, methionine- and glutamine-free DMEM (Gibco, CA, USA) with 100 M methionine, 4 mM glutamine, 1 mM sodium pyruvate, 0.2% bovine serum albumin (BSA), 100 U/ml penicillin and 100 g/ml streptomycin

Thakor AS, Richter HG, Kane AD, Dunster C, Kelly FJ, Poston L, Giussani DA

Fixed ratio: 62.5% GHK-Cu / 12.5% each BPC-157, KPV and TB-500 components share one vial and are not independently adjustable

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