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For cell number measurements in 6-well plates, the medium was aspirated, the wells were rinsed with PBS and the cells were detached by incubating with 500 l of 0.25% trypsin (Thermo Scientific, 25200056) for 20 min at 37 C

SLCs has become a prominent strategy for brain drug delivery of nanoparticles by functionalization with ligands such as hexoses, amino acids or vitamins [18]

However, its angiogenic properties which support blood vessel formationcould theoretically present risks in individuals with undiagnosed cancer or abnormal cell growth

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