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In order to ensure the accuracy of the results, the adjacency and maximum likelihood methods were both deployed, with the LG + G model used for the maximum likelihood method

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Briefly, recombinant PDI protein (0.4 M) was incubated with indicated compounds at 37 C for 1 h in sodium phosphate buffer (100 mM sodium phosphate, 2 mM EDTA and 8 M DTT, pH 7.0)

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