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The membranes were incubated with 5% BSA at room temperature for 1 h, then with primary antibodies at 4 C overnight and second antibody successively for 1 h at room temperature, and consequently developed on the imaging system after adding ECL reagent

Murina, F., Graziottin, A., Felice, R

This case thus exhibits reported karyotypic changes overlapping MMNST, MPNST, and schwannoma (8q gain, and 1p, 17p, and 22q loss), and MMNST and melanoma (6p and 8q gains) (Figure 5A)