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First, the mechanical agitation subjects peptide chains to shear forces at the liquid-air interface
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The 12-week rotation protocol Week 1: Right abdomen, upper quadrant (above navel, right side) Week 2: Left abdomen, upper quadrant (above navel, left side) Week 3: Right anterior thigh, upper portion Week 4: Right abdomen, lower quadrant (below navel, right side) Week 5: Left abdomen, lower quadrant (below navel, left side) Week 6: Left anterior thigh, upper portion Week 7: Right abdomen, lateral (right side, toward hip) Week 8: Left abdomen, lateral (left side, toward hip) Week 9: Right anterior thigh, lower portion Week 10: Right upper arm (if partner available) or right abdomen central Week 11: Left anterior thigh, lower portion Week 12: Left upper arm (if partner available) or left abdomen central After week 12, restart the cycle
Daily administration increases the risk of methionine overload, elevated homocysteine, and gastrointestinal side effects without improving fat oxidation beyond what the liver can process
U.et al (2022)