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Bacteriostatic water is supplied in a multiple-dose container from which repeated withdrawals can be made to dilute or dissolve things for administration

The column was pre-equilibrated with 10 column volumes (CV) of 1 Dulbeccos Phosphate Buffered Saline (DPBS) (pH 7.4) (Sigma-Aldrich)/0.05% (v/v) sodium azide (Sigma-Aldrich), before the supernatant was loaded onto the column at a flow rate of (0.52) mL/min

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To provide experimental evidence for the existence of this disulfide and to determine its midpoint redox potential at p H 7.0, strep-MBP-ROXY9 was incubated with different ratios of DTT/dithiane, whichas calculated by the Nernst equationtranslates into redox potentials between 290 and 210 mV at this p H