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If your skin is already sensitive or dry, the combination of alcohol and a needle can lead to localized dermatitis (skin irritation)

One potential mechanism is thought to involve activation of the central nervous system, with the area postrema (AP) and nucleus of the solitary tract (NTS) and the intermediolateral nucleus (IML) of the spinal cord similarly activated by administration of GLP-1RA 29,30

For anti-C1q or anti-NeuN antibody staining, a DAKO EnVision + System (Agilent Technologies, Santa Clara, CA, USA) was used as the second antibody and the signal was detected using tyramide-enhanced fluorescein isothiocyanate (FITC), as for the TSA method

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