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Without it, cells become large and inefficient, leading to persistent exhaustion

At 30 min (for pERK1/2 staining) or 90 min (for c-Fos staining) after injection, mice were transcardially perfused with phosphate buffer including 4% paraformaldehyde and 0.2% picric acid under anesthesia

Throughout the podcast Gary deftly brushes off numerous studies that contradict the CIM and continuously moves the goalposts by saying that the studies were too short, or they werent done well, but he never provides an example of how they should have been done or what was wrong with them (other than funding source, though ironically, he didnt criticize his own studies for funding source)

However, he noted there may be short-term risks

I diet hard and train hard so I like to get bloodwork done 4x a year to check my lipids, CBC, and hormone levels