Immunostaining After organotypic culture or cryosection, the cochlear explants or tissue sections were fixed with 4% PFA, permeabilized with 1% TritonX-100 in PBS, and blocked by incubation in PBT-1 solution (0.1% Triton X-100, 8% donkey serum, 1% bovine serum albumin, and 0.02% sodium azide in PBS) at room temperature for 1 h
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Clinical trial limitations No pineal peptide has undergone the rigorous Phase I through Phase III clinical trial process required for FDA approval
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[9] [10] The most characteristic symptoms of peripheral neuropathy include numbness, tingling, or a "pins and needles" sensation, typically beginning in the toes and feet and potentially progressing upward in a "stocking-glove" distribution