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The cell pellets were cultured in chondrogenic medium (low-glucose Dulbeccos modified Eagle medium containing 0.1 mmol/L ascorbic acid 2-phosphate, 100 nmol dexamethasone, 40 g/mL proline, 100 U/mL penicillin, 100 g/mL streptomycin, and ITS Premix

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Combining these peptides may provide complementary mechanisms for tissue repair: BPC-157 for its trophic and anti-inflammatory effects, and TB-500 for enhanced cell migration and angiogenesis [8]

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The described protocol is standard laboratory practice for working with lyophilized peptides

Instead, angle the needle so the water runs slowly down the inside wall of the glass vial