Singh - Glutathione: A marker and antioxidant for aging - VOLUME 140 - DECEMBER 01, 2002 - ISSUE 6 : P380-381 Jason Allen, Ryan D
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In brief, HCT116 cells were infected with the GeCKO v1 human CRISPR knockout pooled library using lentiviral transduction at a multiplicity of infection of 0.3, and populations stably expressing gRNAs were selected for using puromycin and expanded for 8 days to allow for essential gene drop-out
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