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The mice were housed in conditions were controlled with a temperature range of 2024 C, humidity maintained at 5060%, and a 12-h lightdark cycle, with free access to food and water
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Portions upstream (443 bp) and downstream (338 bp) of the GDH2 gene were amplified and fused with the 5269 bp SAT1 fragment to form a disruption cassette (~6 kb) for GDH2 gene deletion (Supplementary Fig
Aging and Disease, 12 (8), 1879-1897
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