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In short, separation was achieved by a 36 min gradient of mobile phase A (MPA) (acetonitrile(ACN)/water, 1:1, v/v) and mobile phase B (MPB, i-PrOH/ACN/water, 85:10:5, v/v/v) both containing 5 mM NH 4 HCO 2 and 0.1% formic acid at a flow rate of 300 L/min, a column oven temperature of 50 C, and an injection volume of 5 L

Notably, the usage of vitamin B12 (cyanocobalamin), methylcobalamin, and alpha-lipoic acid can help alleviate nerve discomfort and contribute to an improved quality of life

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(PubMed) Scarpini E, Sacilotto G, Baron P, Cusini M, Scarlato G