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Each PCR was performed in a 20 l reaction mixture containing 2X KAPA SYBR FAST qPCR Master Mix (KAPA Biosystems)
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pH Determination in Water and Culture Medium GHK, GHK-Cu, Cu(OAc) 2 and CuCl 2 samples were prepared and diluted in sterile water and culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin) respectively to a final concentration of 580 M and 5800 M
Why the long off phase: the 4 month pause is built into the protocol
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