The immunoreactive proteins were visualised using horseradish peroxidase-coupled secondary antibodies (Thermo Scientific) and enhanced chemiluminescence reagents according to the manufacturer's instructions (ECL Select Western Blotting Detection Reagent RPN 2235, GE Healthcare, Amersham)
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Instead of appetite surging from zero to maximum in days, a slow taper lets your body relearn hunger cues at a manageable pace
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