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Tirzepatide's dual GLP-1/GIP mechanism produces lower diarrhea rates (~23%) than retatrutide's triple agonist approach

Kicqstart primers for TBP, KEAP1, NFE2L2, SRXN1 and SQSTM1 (Sigma Aldrich, Zwijndrecht, The Netherlands) were used to detect mRNA levels using POWRUP SYBR master mix (Thermo Scientific, Leiden, The Netherlands)

In preclinical research models, the timeline for metabolic response will depend on species, dose, and the specific endpoints being measured

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