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For cell cycle analysis of cells embedded in soft agar, cells (10 5 ) were suspended in 1 mL of 0.33% noble agar, seeded in the bottom of 15-mL polypropylene tubes, cooled for 5 min on ice, and then kept at 37 C in a humidified incubator in the presence of 5% CO 2 for 24 h72 h

These exosomal miR-522 molecules directly bind to the 3'UTR of ALOX15 mRNA, inhibiting its translation and thereby reducing the accumulation of ROS, which leads to cisplatin/paclitaxel resistance

First, the causal direction in human studies remains ambiguous: it is unclear whether gut dysbiosis is a driver of OA or a consequence of its associated factors, such as reduced physical activity, diet changes, or chronic medication use

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& Quinn, J