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Thus, higher GSSG/GSH ratio indicates increased oxidative stress
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Sample volumes were normalized to 35 L with EPPS buffer (200 mM, pH 8), diluted with HPLC grade CH 3 CN (9 L), and then labeled (5 L of 20 g/L per sample) with the corresponding TMTsixplex Isobaric Mass Tag (Themo Scientific, #90064B)
Without adequate collagen, these tissues lose their integrity, leading to joint discomfort, longer recovery times, and visible signs of aging like fine lines
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