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(5) M V Graf, A J Kastin, D H Coy, and A J Fischman (1985) Delta-sleep-inducing peptide reduces CRF-induced corticosterone release Neuroendocrinology, 1985 Oct, Volume 41 (Issue 4), Pages 353-6

Prior to permeability experiments, cells were washed with transport buffer (0.12 M NaCl, 25 mM NaHCO 3 , 3 mM KCl, 2 mM MgSO 4 , 2 mM CaCl 2 , 0.4 mM K 2 HPO 4 , 1 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), and 0.1% human platelet poor derived serum) and 100 L of the molecule of interest in transport buffer was introduced into the apical well

serious events have not been documented in limited human observations