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Following the injection, in vivo imaging was conducted using an IVIS Spectrum (PerkinElmer) at various time points: 1 h, 2 h, 4 h, 6 h, 8 h, 12 h, 24 h, and 48 h post-injection, utilizing an excitation wavelength of 780 nm and a fluorescence emission wavelength of 840 nm

Appropriate secondary antibodies conjugated to Alexa-Fluor 488, 555 or 633 (Supplementary Table 1) were incubated at 1:300 for 2 h before mounting on microscope slides using Prolong Gold

Leukoencephalopathy with accumulated succinate is indicative of SDHAF1 related complex II deficiency

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ROS are oxygen molecules that can damage cells if not balanced

Patients should avoid ingesting large amounts of vitamin C within 1 hour of oral vitamin B12 administration Extended release potassium products Antibiotics such as gentamicin, neomycin, tobramycin Anti-seizure medications such as phenobarbital, phenytoin, primidone Medicines used to treat heartburn like H2 blockers such as cimetidine and proton pump inhibitors such as omeprazole Antacids such as ranitidine, cimetidine Vitamin B12 Injection Storage and Safety Information Vitamin B12 tablets should be in a cool and dry place