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Post-Reconstitution Handling: Store reconstituted solution at 28C Use within a short, validated timeframe based on experimental requirements For extended storage, aliquot and store at -20C or below Avoid repeated freeze-thaw cycles Pre-Reconstitution Storage: Store lyophilized product at 28C (short-term) For long-term storage, maintain at -20C or below Protect from light, heat, and moisture Handling Precautions: Use appropriate aseptic laboratory techniques Allow vial to reach room temperature before opening to prevent condensation Ensure solutions are clear and free of particulates prior to use Dispose of materials in accordance with laboratory safety protocols Research Protocols and Dosing Considerations Research involving L-Glutathione (GSH) varies widely depending on experimental model, cell type, and study objectives

ExpiCHO-S cells were transfected with pCAGGS-AIM with each mutation and cultured in ExpiCHO Expression Medium supplemented with ExpiCHOFeed and ExpiFectamine CHO Enhancer for 4 days in a 37 C incubator with a humidified atmosphere of 8% CO 2 in air on an orbital shaker

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SI: Formal Analysis, Investigation, Methodology, Validation, Visualization, Writing original draft, Writing review and editing

it demands a "Thiol-Lock" strategy

Others use recovery IVs only when needed