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After treatment, the cells were washed with cold PBS and lysed in RIPA buffer (Biosesang, Republic of Korea) supplemented with a protease inhibitor cocktail (Merck, Darmstadt, Germany)

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5-HT, 5-hydroxytryptamine

Several studies suggest that insulin resistance may play a significant role in fibromyalgia including one which associated it with memory issues

Documenting baseline measurements and taking progress photos every 2-4 weeks provides objective evidence of change that daily mirror checks often miss