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Sequencing libraries were generated using NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA) following manufacturers recommendations and index codes were added to attribute sequences to each sample

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[7] These findings suggest that semaglutide improves insulin resistance primarily by reducing adiposity, thereby alleviating metabolic stressors such as ectopic lipid accumulation, inflammatory signaling, and altered adipokine profiles that impair insulin action