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The PCR products were digested with Nco I and Bam HI and ligated into the pKM260 expression vector (EUROSCARF, Frankfurt, Germany), which resulted in the N-terminal addition of a hexahistidine tag and linker with the sequence MHHHHHHASENLYFQGAM
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FIGURE 2 This oxidative stress environment can activate immune signalling pathways promoting neuroinflammation through mitochondrial dysfunction and impaired mitophagy, as observed in recent studies on ASD brains (Pinto Payares et al., 2024)
& Bloomgren, G
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