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To stop the reaction, stop buffer (340 mM NaCl, 20 mM EDTA, 4 mM EGTA, 50 g/mL RNase A, 100 g/mL glycogen, and 3 pg/mL Drosophila DNA) was added, followed by incubation for 3 min at 4 C and for 39 min at 37 C

Flexible to be used on delicate areas such as the eyebrows or upper lip

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( TIR)

1-MX is an adenosine receptor antagonist and increases the release and survivability of neurotransmitters

To test whether these bands represented non-secretin proteins cross-reacting with the antibody, identical protein samples were separated on SDS gels