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2011)were the first to demonstrate region-specific alterations in the oxidative damage marker 3-nitrotyrosine (3-NT) in postmortem brains of individuals with autism, showing that regions such as the orbitofrontal cortex, Wernickes area, the cerebellar vermis, and the cerebellar hemispheres exhibited distinct levels of this biomarker, reflecting region-specific redox signatures

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Stable CHOFlpIn expression cell lines were selected using 600 g/ml hygromyocin B, and maintained in DMEM supplemented with 5% (V/V) FBS (Invitrogen) at 37 C in 5% CO 2