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Liver myeloperoxidase activity To determine liver myeloperoxidase (MPO) activity, liver tissue was homogenized in 1 ml of cold hexdecyltrimethylammonium bromide buffer, sonicated on ice for 10 s and centrifuged at 14,000xg for 30 min at 4 C
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This interaction promotes M2 macrophage polarization, enhancing pro-tumorigenic phenotypes ( 4.3 Macrophage-ferroptosis immunometabolism Tumor-associated macrophages (TAMs) and ferroptotic cancer cells engage in a highly integrated, bidirectional metabolic dialogue that shapes immune outcomes and determines tumor ( + , increased transferrin receptor (TfR1) expression, and restricted ferroportin activity, thereby enriching the tumor microenvironment (TME) with redox-active iron ( Ferroptotic cancer cells reciprocally influence macrophage states by releasing DAMPs (HMGB1), oxidized phosphatidylethanolamines (SAPE-OOH), iron-loaded vesicles, and lipid peroxidation products that serve as signals to recruit, polarize, or reprogram macrophages ( Figure 5
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