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The samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose (NC) membranes (GE Healthcare)

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Glutamine maintains neutrality as an amino acid while glutamate becomes negatively charged when deaminated

The degree of phase II conjugation, typically encompassing sulfation, glucuronidation and methylation (see section 6.1 Pre-systemic metabolism: role of gut microbiota), is high for individual molecules (Table 3)

Fortunately, glutathione helps regulate glutamate and protect your neurons

& Jager, K